site-directedmutagenesis相关论文
Characterization of the First Fungal Glycosyl Hydrolase Family 19 Chitinase(NbchiA)from Nosema bomby
Chitinases(EC 3.2.1.14),as one kind of glycosyl hydrolase,hydrolyze the β-(1,4)linkages of chitin.According to the sequ......
Benzimidazole resistance of Gibberella zeae.(anonmorph: Fusarium graminearum) was conferred by point mutation in β2-tub......
Escherichia coli phytase appA belongs to the histidine acid phosphatase family.With a high hydrolytic activity towards p......
Heparan sulfate (HS) is an abundantpolysaccharide in the animal kingdom withessential physiological functions.HS is comp......
Heparansulfate (HS) is an abundantpolysaccharide in the animal kingdom withessential physiological functions.HS is compo......
Biochemical Properties of Isocitrate Dehydrogenase from Streptococcus mutans and Functional Evaluati
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The aerolysin nanopore channel is one of the confined spaces for single molecule analysis which displays high spatial an......
Characterization of Catalytic Activity and Structure of Selenocysteine-Containing hGSTZ1c-1c Based o
Human glutathione transferase zeta 1c-1c (hGSTZ1c-1c) is one of the glutathione transferase isoenzymes and considered to......
Molecular Basis for the High Activity and Enantioselectivity of the Carbonyl Reductase from Sporobol
In an effort to develop a practical method for the synthesis of optically pure 2,2,2-trifluoro-1-phenylethanol,we fo......
Primer SpannerA Web-based Platform to Design PCR Primers for High Efficient Site-directed Mutagenesi
Background: PCR-based strategy has economical and efficient application in site-directed mutagenesis(SDM)and DNA ass......
A further identification of redox-sensitive control of kinase activities of OsMPK3 and OsMPK6 in vit
Two subgroup A MAPKs,MPK3 and MPK6,have been shown to be conserved and ubiquitously implicated in multiple stress respon......
Functional analysis of an extral cysteine in the repetitive domain of HMW-GS in common wheat (Tritic
Processing quality of wheat is largely due to viscoelasticity of wheat gluten, the elasticity of which is mainly determi......
The thermal stability and catalytic activity of endoglucanase(EngD)from thermophilic Thermotoga maritima were signif......
Single-Point Mutations (R153H or R153C) in NADP+-Dependent Isocitrate Dehydrogenases from Escherichi
Arginine 132 (R132) mutations to histidine or cysteine frequently occur to cytosolic NADP+-isocitrate dehydrogenase ......
Isocitrate dehydrogenase (IDH), as an old housekeeping gene and a popular star, drawing more and more attention of c......
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The site-directed and saturation mutagenesis methods are very important to probe the structure-function relationship for......
Analysis of essential carboxylic amino acid residues for catalytic activity of Aspergillus sp.CJ22-3
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Structure-based analysis of the ligand binding mechanism for the DhelOBP21,a C-Minus odorant binding
Odorant binding proteins(OBPs)transport hydrophobic odor molecules across the sensillar lymph to trigger a neuronal ......
Vascular proliferation is one of the major causes for morbidity and mortality in diabetes. However, the cellular and mol......
CD59 may inhibit the cytolytic activity of complement by binding to C8/C9 and protect host cell membranes against homolo......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
验证血红素加氧酶－１中的第３９位赖氨酸对电子传递的影响。方法通过定眯诱变将Ｋ３９置换为酸性氨基酸谷氨酸（Ｅ）将将得到的变异型酶基因克隆到高度......
有机磷和氨基甲酸酯类杀虫剂抑制乙酰胆碱酯酶（AChE）的天然生物活性。近年来，AChE作为生物识别元素的生物传感器在杀虫剂残留量检测领......
